Combining microscopy with mesoscopy using optical and optoacoustic label-free modes
Soliman D, Tserevelakis GJ, Omar M, Ntziachristos V (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 5
Article Number: 12902
DOI: 10.1038/srep12902
Abstract
Biology requires observations at multiple geometrical scales, a feature that is not typically offered by a single imaging modality. We developed a hybrid optical system that not only provides different contrast modes but also offers imaging at different geometrical scales, achieving uniquely broad resolution and a 1000-fold volume sampling increase compared to volumes scanned by optical microscopy. The system combines optoacoustic mesoscopy, optoacoustic microscopy and two-photon microscopy, the latter integrating second and third harmonic generation modes. Label-free imaging of a mouse ear and zebrafish larva ex-vivo demonstrates the contrast and scale complementarity provided by the hybrid system. We showcase the superior anatomical orientation offered by the label-free capacity and hybrid operation, over fluorescence microscopy, and the dynamic selection between field of view and resolution achieved, leading to new possibilities in biological visualization.
Involved external institutions
Germany (DE)How to cite
APA:
Soliman, D., Tserevelakis, G.J., Omar, M., & Ntziachristos, V. (2015). Combining microscopy with mesoscopy using optical and optoacoustic label-free modes. Scientific Reports, 5. https://doi.org/10.1038/srep12902
MLA:
Soliman, Dominik, et al. "Combining microscopy with mesoscopy using optical and optoacoustic label-free modes." Scientific Reports 5 (2015).
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