Selective plane illumination optical and optoacoustic microscopy for postembryonic imaging
Lin HCA, Chekkoury A, Omar M, Schmitt-Manderbach T, Koberstein-Schwarz B, Mappes T, Lopez-Schier H, Razansky D, Ntziachristos V (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 9
Pages Range: L29-L34
Journal Issue: 5
Abstract
Intravital imaging of large specimens is intrinsically challenging for postembryonic studies. Selective plane illumination microscopy (SPIM) has been introduced to volumetrically visualize organisms used in developmental biology and experimental genetics. Ideally suited for imaging transparent samples, SPIM can offer high frame rate imaging with optical microscopy resolutions and low phototoxicity. However, its performance quickly deteriorates when applied to opaque tissues. To overcome this limitation, SPIM optics were merged with optical and optoacoustic (photoacoustic) readouts. The performance of this hybrid imaging system was characterized using various phantoms and by imaging a highly scattering ex vivo juvenile zebrafish. The results revealed the system's enhanced capability over that of conventional SPIM for high-resolution imaging over extended depths of scattering content. The approach described here may enable future visualization of organisms throughout their entire development, encompassing regimes in which the tissue may become opaque.
Involved external institutions
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Carl Zeiss AG
Germany (DE)
Germany (DE)
Carl Zeiss AG
Germany (DE)
How to cite
APA:
Lin, H.-C.A., Chekkoury, A., Omar, M., Schmitt-Manderbach, T., Koberstein-Schwarz, B., Mappes, T.,... Ntziachristos, V. (2015). Selective plane illumination optical and optoacoustic microscopy for postembryonic imaging. Laser & Photonics Reviews, 9(5), L29-L34. https://doi.org/10.1002/lpor.201500120
MLA:
Lin, Hsiao-Chun Amy, et al. "Selective plane illumination optical and optoacoustic microscopy for postembryonic imaging." Laser & Photonics Reviews 9.5 (2015): L29-L34.
BibTeX: Download