Light fluence normalization in turbid tissues via temporally unmixed multispectral optoacoustic tomography
Dean-Ben XL, Stiel AC, Jiang Y, Ntziachristos V, Westmeyer GG, Razansky D (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 40
Pages Range: 4691-4694
Journal Issue: 20
DOI: 10.1364/OL.40.004691
Abstract
Discerning the accurate distribution of chromophores and biomarkers by means of optoacoustic imaging is commonly challenged by the highly heterogeneous excitation light patterns resulting from strong spatial variations of tissue scattering and absorption. Here we used the light-fluence dependent switching kinetics of reversibly switchable fluorescent proteins (RSFPs), in combination with real-time acquisition of volumetric multi-spectral optoacoustic data to correct for the light fluence distribution deep in scattering media. The new approach allows for dynamic fluence correction in time-resolved imaging, e.g., of moving organs, and can be extended to work with a large palette of available synthetic and genetically encoded photochromic substances for multiplexed wavelength-specific fluence normalization.
Involved external institutions
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Max-Planck-Institut für Entwicklungsbiologie / Max Planck Institute for Developmental Biology
Germany (DE)
Germany (DE)
Max-Planck-Institut für Entwicklungsbiologie / Max Planck Institute for Developmental Biology
Germany (DE)
How to cite
APA:
Dean-Ben, X.L., Stiel, A.C., Jiang, Y., Ntziachristos, V., Westmeyer, G.G., & Razansky, D. (2015). Light fluence normalization in turbid tissues via temporally unmixed multispectral optoacoustic tomography. Optics Letters, 40(20), 4691-4694. https://doi.org/10.1364/OL.40.004691
MLA:
Dean-Ben, X. Luis, et al. "Light fluence normalization in turbid tissues via temporally unmixed multispectral optoacoustic tomography." Optics Letters 40.20 (2015): 4691-4694.
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