Rieken S, Rieber J, Brons S, Habermehl D, Rief H, Orschiedt L, Lindel K, Weber KJ, Debus J, Combs SE (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 56
Pages Range: 430-436
Journal Issue: 3
DOI: 10.1093/jrr/rru120
Photon irradiation has been repeatedly suspected of increasing tumor cell motility and promoting locoregional recurrence of disease. This study was set up to analyse possible mechanisms underlying the potentially radiation-altered motility in medulloblastoma cells. Medulloblastoma cell lines D425 and Med8A were analyzed in migration and adhesion experiments with and without photon and carbon ion irradiation. Expression of integrins was determined by quantitative FACS analysis. Matrix metalloproteinase concentrations within cell culture supernatants were investigated by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using Student's t-test. Both photon and carbon ion irradiation significantly reduced chemotactic medulloblastoma cell transmigration through 8-μm pore size membranes, while simultaneously increasing adherence to fibronectin- and collagen I- and IV-coated surfaces. Correspondingly, both photon and carbon ion irradiation downregulate soluble MMP9 concentrations, while upregulating cell surface expression of proadhesive extracellular matrix protein-binding integrin α
APA:
Rieken, S., Rieber, J., Brons, S., Habermehl, D., Rief, H., Orschiedt, L.,... Combs, S.E. (2015). Radiation-induced motility alterations in medulloblastoma cells. Journal of Radiation Research, 56(3), 430-436. https://doi.org/10.1093/jrr/rru120
MLA:
Rieken, Stefan, et al. "Radiation-induced motility alterations in medulloblastoma cells." Journal of Radiation Research 56.3 (2015): 430-436.
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