Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy

Omar M, Rebling J, Wicker K, Schmitt-Manderbach T, Schwarz M, Gateau J, Lopez-Schier H, Mappes T, Ntziachristos V (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Light: Science & Applications CIOMP

Book Volume: 6

Article Number: e16186

Journal Issue: 1

DOI: 10.1038/lsa.2016.186

Abstract

Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal. It requires a combination of high-resolution performance and high-penetration depth. Optoacoustic (photoacoustic) mesoscopy holds great promise, as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution. However, optoacoustic mesoscopic techniques only offer partial visibility of oriented structures, such as blood vessels, due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution. We introduce 360° multi orientation (multi-projection) raster scan optoacoustic mesoscopy (MORSOM) based on detecting an ultra-wide frequency bandwidth (up to 160 MHz) and weighted deconvolution to synthetically enlarge the angular aperture. We report unprecedented isotropic in-plane resolution at the 9-17 μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish. We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms, with possible applications in the developmental biology of adulthood and aging.

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) Carl Zeiss AG DE Germany (DE) PSL Research University / Université de recherche Paris Sciences et Lettres FR France (FR) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Carl Zeiss Vision GmbH DE Germany (DE)

How to cite

APA:

Omar, M., Rebling, J., Wicker, K., Schmitt-Manderbach, T., Schwarz, M., Gateau, J.,... Ntziachristos, V. (2017). Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy. Light: Science & Applications, 6(1). https://doi.org/10.1038/lsa.2016.186

MLA:

Omar, Murad, et al. "Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy." Light: Science & Applications 6.1 (2017).

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