Bozhko D, Osborn EA, Rosenthal A, Verjans JW, Hara T, Kellnberger S, Wissmeyer G, Ovsepian SV, Mccarthy JR, Mauskapf A, Stein AF, Jaffer FA, Ntziachristos V (2017)
Publication Type: Journal article
Publication year: 2017
Book Volume: 18
Pages Range: 1253-1261
Journal Issue: 11
DOI: 10.1093/ehjci/jew222
Aims: (i) to evaluate a novel hybrid near-infrared fluorescence - intravascular ultrasound (NIRF-IVUS) system in coronary and peripheral swine arteries in vivo; (ii) to assess simultaneous quantitative biological and morphological aspects of arterial disease. Methods and results: Two 9F/15MHz peripheral and 4.5F/40MHz coronary near-infrared fluorescence (NIRF)-IVUS catheters were engineered to enable accurate co-registrtation of biological and morphological readings simultaneously in vivo. A correction algorithm utilizing IVUS information was developed to account for the distance-related fluorescence attenuation due to through-blood imaging. Corrected NIRF (cNIRF)-IVUS was applied for in vivo imaging of angioplasty-induced vascular injury in swine peripheral arteries and experimental fibrin deposition on coronary artery stents, and of atheroma in a rabbit aorta, revealing feasibility to intravascularly assay plaque structure and inflammation. The addition of ICG-enhanced NIRF assessment improved the detection of angioplasty-induced endothelial damage compared to standalone IVUS. In addition, NIRF detection of coronary stent fibrin by in vivo cNIRF-IVUS imaging illuminated stent pathobiology that was concealed on standalone IVUS. Fluorescence reflectance imaging and microscopy of resected tissues corroborated the in vivo findings. Conclusions: Integrated cNIRF-IVUS enables simultaneous co-registered through-blood imaging of disease related morphological and biological alterations in coronary and peripheral arteries in vivo. Clinical translation of cNIRF-IVUS may significantly enhance knowledge of arterial pathobiology, leading to improvements in clinical diagnosis and prognosis, and helps to guide the development of new therapeutic approaches for arterial diseases.
APA:
Bozhko, D., Osborn, E.A., Rosenthal, A., Verjans, J.W., Hara, T., Kellnberger, S.,... Ntziachristos, V. (2017). Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo. European Heart Journal - Cardiovascular Imaging, 18(11), 1253-1261. https://doi.org/10.1093/ehjci/jew222
MLA:
Bozhko, Dmitry, et al. "Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo." European Heart Journal - Cardiovascular Imaging 18.11 (2017): 1253-1261.
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