Three-dimensional virtual histology enabled through cytoplasm-specific X-ray stain for microscopic and nanoscopic computed tomography
Busse M, Mueller M, Kimm MA, Ferstl S, Allner S, Achterhold K, Herzen J, Pfeiffer F (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 115
Pages Range: 2293-2298
Journal Issue: 10
Abstract
Many histological methods require staining of the cytoplasm, which provides instrumental details for diagnosis. One major limitation is the production of 2D images obtained by destructive preparation of 3D tissue samples. X-ray absorption micro- and nanocomputed tomography (microCT and nanoCT) allows for a nondestructive investigation of a 3D tissue sample, and thus aids to determine regions of interest for further histological examinations. However, application of microCT and nanoCT to biological samples (e.g., biopsies) is limited by the missing contrast within soft tissue, which is important to visualize morphological details. We describe an eosin-based preparation overcoming the challenges of contrast enhancement and selectivity for certain tissues. The eosin-based staining protocol is suitable for whole-organ staining, which then enables high-resolution microCT imaging of whole organs and nanoCT imaging of smaller tissue pieces retrieved from the original sample. Our results demonstrate suitability of the eosin-based staining method for diagnostic screening of 3D tissue samples without impeding further diagnostics through histological methods.
Involved external institutions
Germany (DE)How to cite
APA:
Busse, M., Mueller, M., Kimm, M.A., Ferstl, S., Allner, S., Achterhold, K.,... Pfeiffer, F. (2018). Three-dimensional virtual histology enabled through cytoplasm-specific X-ray stain for microscopic and nanoscopic computed tomography. Proceedings of the National Academy of Sciences of the United States of America, 115(10), 2293-2298. https://doi.org/10.1073/pnas.1720862115
MLA:
Busse, Madleen, et al. "Three-dimensional virtual histology enabled through cytoplasm-specific X-ray stain for microscopic and nanoscopic computed tomography." Proceedings of the National Academy of Sciences of the United States of America 115.10 (2018): 2293-2298.
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