Structure-Based Mutagenesis of Phycobiliprotein smURFP for Optoacoustic Imaging

Werner JPF, Mishra K, Huang Y, Vetschera P, Glasl S, Chmyrov A, Richter K, Ntziachristos V, Stiel AC (2019)

Publication Type: Journal article

Publication year: 2019

Journal

ACS Chemical Biology American Chemical Society

Book Volume: 14

Pages Range: 1896-1903

Journal Issue: 9

DOI: 10.1021/acschembio.9b00299

Abstract

Photo- or optoacoustics (OA) imaging is increasingly being used as a non-invasive imaging method that can simultaneously reveal structure and function in deep tissue. However, the most frequent transgenic OA labels are current fluorescent proteins that are not optimized for OA imaging. Thus, they lack OA signal strength, and their absorption maxima are positioned at short wavelengths, thus giving small penetration depths and strong background signals. Here, we apply insights from our recent determination of the structure of the fluorescent phycobiliprotein smURFP to mutate a range of residues to promote the nonradiative decay pathway that generates the OA signal. We identified hydrophobic and aromatic substitutions within the chromophore-binding pocket that substantially increase the intensity of the OA signal and red-shift the absorption. Our results demonstrate the feasibility of structure-based mutagenesis to repurpose fluorescent probes for OA imaging, and they may provide structure-function insights for de novo engineering of transgenic OA probes.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Werner, J.P.F., Mishra, K., Huang, Y., Vetschera, P., Glasl, S., Chmyrov, A.,... Stiel, A.C. (2019). Structure-Based Mutagenesis of Phycobiliprotein smURFP for Optoacoustic Imaging. ACS Chemical Biology, 14(9), 1896-1903. https://doi.org/10.1021/acschembio.9b00299

MLA:

Werner, Juan Pablo Fuenzalida, et al. "Structure-Based Mutagenesis of Phycobiliprotein smURFP for Optoacoustic Imaging." ACS Chemical Biology 14.9 (2019): 1896-1903.

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