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Quantifying Structural Heterogeneity of Healthy and Cancerous Mitochondria Using a Combined Segmentation and Classification USK-Net

Mishra M, Schmitt S, Zischka H, Strasser M, Navab N, Marr C, Peng T (2019)

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Publication Type: Conference contribution

Publication year: 2019

Journal

Publisher: Springer Verlag

Book Volume: 11731 LNCS

Pages Range: 289-298

Conference Proceedings Title: Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)

Event location: Munich, DEU

ISBN: 9783030304928

DOI: 10.1007/978-3-030-30493-5_30

Abstract

Mitochondria are the main source of cellular energy and thus essential for cell survival. Pathological conditions like cancer, can cause functional alterations and lead to mitochondrial dysfunction. Indeed, electron micrographs of mitochondria that are isolated from cancer cells show a different morphology as compared to mitochondria from healthy cells. However, the description of mitochondrial morphology and the classification of the respective samples are so far qualitative. Furthermore, large intra-class variability and impurities such as mitochondrial fragments and other organelles in the micrographs make a clear separation between healthy and cancerous samples challenging. In this study, we propose a deep-learning based model to quantitatively assess the status of each intact mitochondrion with a continuous score, which measures its closeness to the healthy/tumor classes based on its morphology. This allows us to describe the structural transition from healthy to cancerous mitochondria. Methodologically, we train two USK networks, one to segment individual mitochondria from an electron micrograph, and the other to softly classify each image pixel as belonging to (i) healthy mitochondrial, (ii) cancerous mitochondrial and (iii) non-mitochondrial (image background & impurities) tissue. Our combined model outperforms each network alone in both pixel classification and object segmentation. Moreover, our model can quantitatively assess the mitochondrial heterogeneity within and between healthy samples and different tumor types, hence providing insightful information of mitochondrial alterations in cancer development.

Involved external institutions

How to cite

APA:

Mishra, M., Schmitt, S., Zischka, H., Strasser, M., Navab, N., Marr, C., & Peng, T. (2019). Quantifying Structural Heterogeneity of Healthy and Cancerous Mitochondria Using a Combined Segmentation and Classification USK-Net. In Vera Kurková, Igor V. Tetko, Pavel Karpov, Fabian Theis (Eds.), Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) (pp. 289-298). Munich, DEU: Springer Verlag.

MLA:

Mishra, Manish, et al. "Quantifying Structural Heterogeneity of Healthy and Cancerous Mitochondria Using a Combined Segmentation and Classification USK-Net." Proceedings of the 28th International Conference on Artificial Neural Networks, ICANN 2019, Munich, DEU Ed. Vera Kurková, Igor V. Tetko, Pavel Karpov, Fabian Theis, Springer Verlag, 2019. 289-298.

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