Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells

Pleitez MA, Khan AA, Solda A, Chmyrov A, Reber J, Gasparin F, Seeger MR, Schaetz B, Herzig S, Scheideler M, Ntziachristos V (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Nature Biotechnology Nature Publishing Group

Book Volume: 38

Pages Range: 293-296

Journal Issue: 3

DOI: 10.1038/s41587-019-0359-9

Abstract

We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE)

How to cite

APA:

Pleitez, M.A., Khan, A.A., Solda, A., Chmyrov, A., Reber, J., Gasparin, F.,... Ntziachristos, V. (2020). Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells. Nature Biotechnology, 38(3), 293-296. https://doi.org/10.1038/s41587-019-0359-9

MLA:

Pleitez, Miguel A., et al. "Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells." Nature Biotechnology 38.3 (2020): 293-296.

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