Indocyanine green matching phantom for fluorescence-guided surgery imaging system characterization and performance assessment
Ruiz AJ, Wu M, Larochelle EPM, Gorpas D, Ntziachristos V, Pfefer TJ, Pogue BW (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 25
Pages Range: 1-15
Journal Issue: 5
DOI: 10.1117/1.JBO.25.5.056003
Abstract
SIGNIFICANCE: Expanded use of fluorescence-guided surgery with devices approved for use with indocyanine green (ICG) has led to a range of commercial systems available. There is a compelling need to be able to independently characterize system performance and allow for cross-system comparisons. AIM: The goal of this work is to expand on previous proposed fluorescence imaging standard designs to develop a long-term stable phantom that spectrally matches ICG characteristics and utilizes 3D printing technology for incorporating tissue-equivalent materials. APPROACH: A batch of test targets was created to assess ICG concentration sensitivity in the 0.3- to 1000-nM range, tissue-equivalent depth sensitivity down to 6 mm, and spatial resolution with a USAF test chart. Comparisons were completed with a range of systems that have significantly different imaging capabilities and applications, including the Li-Cor® Odyssey, Li-Cor® Pearl, PerkinElmer® Solaris, and Stryker® Spy Elite. RESULTS: Imaging of the ICG-matching phantoms with all four commercially available systems showed the ability to benchmark system performance and allow for cross-system comparisons. The fluorescence tests were able to assess differences in the detectable concentrations of ICG with sensitivity differences >10× for preclinical and clinical systems. Furthermore, the tests successfully assessed system differences in the depth-signal decay rate, as well as resolution performance and image artifacts. The manufacturing variations, photostability, and mechanical design of the tests showed promise in providing long-term stable standards for fluorescence imaging. CONCLUSIONS: The presented ICG-matching phantom provides a major step toward standardizing performance characterization and cross-system comparisons for devices approved for use with ICG. The developed hybrid manufacturing platform can incorporate long-term stable fluorescing agents with 3D printed tissue-equivalent material. Further, long-term testing of the phantom and refinements to the manufacturing process are necessary for future implementation as a widely adopted fluorescence imaging standard.
Involved external institutions
Dartmouth College
United States (USA) (US)
Food and Drug Administration (FDA / USFDA)
United States (USA) (US)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
United States (USA) (US)
Food and Drug Administration (FDA / USFDA)
United States (USA) (US)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
How to cite
APA:
Ruiz, A.J., Wu, M., Larochelle, E.P.M., Gorpas, D., Ntziachristos, V., Pfefer, T.J., & Pogue, B.W. (2020). Indocyanine green matching phantom for fluorescence-guided surgery imaging system characterization and performance assessment. Journal of Biomedical Optics, 25(5), 1-15. https://doi.org/10.1117/1.JBO.25.5.056003
MLA:
Ruiz, Alberto J., et al. "Indocyanine green matching phantom for fluorescence-guided surgery imaging system characterization and performance assessment." Journal of Biomedical Optics 25.5 (2020): 1-15.
BibTeX: Download