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Multispectral optoacoustic tomography of muscle perfusion and oxygenation under arterial and venous occlusion: A human pilot study

Karlas A, Kallmayer M, Fasoula NA, Liapis E, Bariotakis M, Kroenke M, Anastasopoulou M, Reber J, Eckstein HH, Ntziachristos V (2020)

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Publication Type: Journal article

Publication year: 2020

Journal

Book Volume: 13

Article Number: e201960169

Journal Issue: 6

DOI: 10.1002/jbio.201960169

Abstract

Perfusion and oxygenation are critical parameters of muscle metabolism in health and disease. They have been both the target of many studies, in particular using near-infrared spectroscopy (NIRS). However, difficulties with quantifying NIRS signals have limited a wide dissemination of the method to the clinics. Our aim was to investigate whether clinical multispectral optoacoustic tomography (MSOT) could enable the label-free imaging of muscle perfusion and oxygenation under clinically relevant challenges: the arterial and venous occlusion. We employed a hybrid clinical MSOT/ultrasound system equipped with a hand-held scanning probe to visualize hemodynamic and oxygenation changes in skeletal muscle under arterial and venous occlusions. Four (N = 4) healthy volunteers were scanned over the forearm for both 3-minute occlusion challenges. MSOT-recorded pathophysiologically expected results during tests of disturbed blood flow with high resolution and without the need for contrast agents. During arterial occlusion, MSOT-extracted Hb-values showed an increase, while HbO2- and total blood volume (TBV)-values remained roughly steady, followed by a discrete increase during the hyperemic period after cuff deflation. During venous occlusion, results showed a clear increase in intramuscular HbO2, Hb and TBV within the segmented muscle area. MSOT was found to be capable of label-free non-invasive imaging of muscle hemodynamics and oxygenation under arterial and venous occlusion. We introduce herein MSOT as a novel modality for the assessment of vascular disorders characterized by disturbed blood flow, such as acute limb ischemia and venous thrombosis.

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How to cite

APA:

Karlas, A., Kallmayer, M., Fasoula, N.-A., Liapis, E., Bariotakis, M., Kroenke, M.,... Ntziachristos, V. (2020). Multispectral optoacoustic tomography of muscle perfusion and oxygenation under arterial and venous occlusion: A human pilot study. Journal of Biophotonics, 13(6). https://doi.org/10.1002/jbio.201960169

MLA:

Karlas, Angelos, et al. "Multispectral optoacoustic tomography of muscle perfusion and oxygenation under arterial and venous occlusion: A human pilot study." Journal of Biophotonics 13.6 (2020).

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