Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins

Mishra K, Stankevych M, Werner JPF, Grassmann S, Gujrati V, Huang Y, Klemm U, Buchholz VR, Ntziachristos V, Stiel AC (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Science Advances American Association for the Advancement of Science

Book Volume: 6

Article Number: eaaz6293

Journal Issue: 24

DOI: 10.1126/sciadv.aaz6293

Abstract

We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning–based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Mishra, K., Stankevych, M., Werner, J.P.F., Grassmann, S., Gujrati, V., Huang, Y.,... Stiel, A.C. (2020). Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins. Science Advances, 6(24). https://doi.org/10.1126/sciadv.aaz6293

MLA:

Mishra, Kanuj, et al. "Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins." Science Advances 6.24 (2020).

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