Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice

Karlina R, Lutter D, Miok V, Fischer D, Altun I, Schoettl T, Schorpp K, Israel A, Cero C, Johnson JW, Kapser-Fischer I, Boettcher A, Keipert S, Feuchtinger A, Graf E, Strom T, Walch A, Lickert H, Walzthoeni T, Heinig M, Theis FJ, Garcia-Caceres C, Cypess AM, Ussar S (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Life Science Alliance Life Science Alliance

Book Volume: 4

Article Number: e202000924

Journal Issue: 1

DOI: 10.26508/LSA.202000924

Abstract

Brown adipose tissue (BAT) plays an important role in the regulation of body weight and glucose homeostasis. Although increasing evidence supports white adipose tissue heterogeneity, little is known about heterogeneity withinmurine BAT. Recently, UCP1 high and low expressing brown adipocytes were identified, but a developmental origin of these subtypes has not been studied. To obtain more insights into brown preadipocyte heterogeneity, we use single-cell RNA sequencing of the BAT stromal vascular fraction of C57/BL6mice and characterize brown preadipocyte and adipocyte clonal cell lines. Statistical analysis of gene expression profiles from brown preadipocyte and adipocyte clones identify markers distinguishing brown adipocyte subtypes. We confirm the presence of distinct brown adipocyte populations in vivo using the markers EIF5, TCF25, and BIN1. We also demonstrate that loss of Bin1 enhances UCP1 expression and mitochondrial respiration, suggesting that BIN1 marks dormant brown adipocytes. The existence of multiple brown adipocyte subtypes suggests distinct functional properties of BAT depending on its cellular composition, with potentially distinct functions in thermogenesis and the regulation of whole body energy homeostasis.

Involved external institutions

Deutsches Zentrum für Diabetesforschung e.V. (DZD) DE Germany (DE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) US National Institutes of Health (NIH) US United States (USA) (US) Stockholm University / Stockholms universitet SE Sweden (SE)

How to cite

APA:

Karlina, R., Lutter, D., Miok, V., Fischer, D., Altun, I., Schoettl, T.,... Ussar, S. (2021). Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice. Life Science Alliance, 4(1). https://doi.org/10.26508/LSA.202000924

MLA:

Karlina, Ruth, et al. "Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice." Life Science Alliance 4.1 (2021).

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