3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents
Busse M, Marciniszyn JP, Ferstl S, Kimm MA, Pfeiffer F, Gulder T (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 27
Pages Range: 4561-4566
Journal Issue: 14
Abstract
Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X-ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X-ray computed tomography imaging (CT) to enhance the soft tissue X-ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X-ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.
Involved external institutions
Germany (DE)How to cite
APA:
Busse, M., Marciniszyn, J.P., Ferstl, S., Kimm, M.A., Pfeiffer, F., & Gulder, T. (2021). 3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents. Chemistry - A European Journal, 27(14), 4561-4566. https://doi.org/10.1002/chem.202005203
MLA:
Busse, Madleen, et al. "3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents." Chemistry - A European Journal 27.14 (2021): 4561-4566.
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