CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett’s esophagus

Marcazzan S, Carvalho MJB, Konrad M, Strangmann J, Tenditnaya A, Baumeister T, Schmid RM, Wester HJ, Ntziachristos V, Gorpas D, Wang TC, Schottelius M, Quante M (2022)

Publication Type: Journal article

Publication year: 2022

Journal

EJNMMI Research SpringerOpen / BioMed Central

Book Volume: 12

Article Number: 2

Journal Issue: 1

DOI: 10.1186/s13550-021-00875-7

Abstract

Background: Near-infrared (NIR) fluorescence imaging has been emerging as a promising strategy to overcome the high number of early esophageal adenocarcinomas missed by white light endoscopy and random biopsy collection. We performed a preclinical assessment of fluorescence imaging and endoscopy using a novel CXCR4-targeted fluorescent peptide ligand in the L2-IL1B mouse model of Barrett’s esophagus. Methods: Six L2-IL1B mice with advanced stage of disease (12–16 months old) were injected with the CXCR4-targeted, Sulfo-Cy5-labeled peptide (MK007), and ex vivo wide-field imaging of the whole stomach was performed 4 h after injection. Before ex vivo imaging, fluorescence endoscopy was performed in three L2-IL1B mice (12–14 months old) by a novel imaging system with two L2-IL1B mice used as negative controls. Results: Ex vivo imaging and endoscopy in L2-IL1B mice showed that the CXCR4-targeted MK007 accumulated mostly in the dysplastic lesions with a mean target-to-background ratio > 2. The detection of the Sulfo-Cy5 signal in dysplastic lesions and its co-localization with CXCR4 stained cells by confocal microscopy further confirmed the imaging results. Conclusions: This preliminary preclinical study shows that CXCR4-targeted fluorescence endoscopy using MK007 can detect dysplastic lesions in a mouse model of Barrett’s esophagus. Further investigations are needed to assess its use in the clinical setting.

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) Columbia University US United States (USA) (US)

How to cite

APA:

Marcazzan, S., Carvalho, M.J.B., Konrad, M., Strangmann, J., Tenditnaya, A., Baumeister, T.,... Quante, M. (2022). CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett’s esophagus. EJNMMI Research, 12(1). https://doi.org/10.1186/s13550-021-00875-7

MLA:

Marcazzan, Sabrina, et al. "CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett’s esophagus." EJNMMI Research 12.1 (2022).

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