Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy
Visscher M, Pleitez MA, Van Gaalen K, Nieuwenhuizen-Bakker IM, Ntziachristos V, Van Soest G (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 26
Article Number: 100354
DOI: 10.1016/j.pacs.2022.100354
Abstract
Background and aims: Analysis of atherosclerotic plaque composition is a vital tool for unraveling the pathological metabolic processes that contribute to plaque growth. Methods: We visualize the constitution of human carotid plaques by mid-infrared optoacoustic microscopy (MiROM), a method for label-free analytic histology that requires minimal tissue preparation, rapidly yielding large field-of-view en-face images with a resolution of a few micrometers. We imaged endarterectomy specimens (n = 3, 12 sections total) at specific vibrational modes, targeting carbohydrates, lipids and proteins. Additionally, we recorded spectra at selected tissue locations. We identified correlations in the variability in this high-dimensional data set using non-negative matrix factorization (NMF). Results: We visualized high-risk plaque features with molecular assignment. Consistent NMF components relate to different dominant tissue constituents, dominated by lipids, proteins, and cholesterol and carbohydrates respectively. Conclusions: These results introduce MiROM as an innovative, stain-free, analytic histology technology for the biochemical characterization of complex human vascular pathology.
Involved external institutions
Erasmus University Medical Center (MC)
Netherlands (NL)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Netherlands (NL)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
How to cite
APA:
Visscher, M., Pleitez, M.A., Van Gaalen, K., Nieuwenhuizen-Bakker, I.M., Ntziachristos, V., & Van Soest, G. (2022). Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy. Photoacoustics, 26. https://doi.org/10.1016/j.pacs.2022.100354
MLA:
Visscher, Mirjam, et al. "Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy." Photoacoustics 26 (2022).
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