Zhang F, Meier ABB, Lipp P, Laugwitz KL, Dorn T, Moretti A (2022)
Publication Type: Journal article
Publication year: 2022
Book Volume: 60
Article Number: 102731
DOI: 10.1016/j.scr.2022.102731
TRPM4 is a Ca2+-activated channel mediating the transport of monovalent cations across the cell membrane. Mutations in the TRPM4 gene have been associated with cardiac arrhythmias in humans. Using CRISPR/Cas9 gene editing technology, we established two TRPM4 knockout human iPSC lines – one heterozygous (MRli003-A-3) and one homozygous (MRli003-A-4) – by inserting a frameshift mutation in exon 2 of the TRPM4 gene. Both lines maintained pluripotency, a normal karyotype, parental cell morphology, and the ability to differentiate into the three germ layers.
APA:
Zhang, F., Meier, A.B.B., Lipp, P., Laugwitz, K.-L., Dorn, T., & Moretti, A. (2022). Generation of heterozygous (MRli003-A-3) and homozygous (MRli003-A-4) TRPM4 knockout human iPSC lines. Stem Cell Research, 60. https://doi.org/10.1016/j.scr.2022.102731
MLA:
Zhang, Fangfang, et al. "Generation of heterozygous (MRli003-A-3) and homozygous (MRli003-A-4) TRPM4 knockout human iPSC lines." Stem Cell Research 60 (2022).
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