Marathon-induced cardiac strain as model for the evaluation of diagnostic micrornas for acute myocardial infarction

Shirvani Samani O, Scherr J, Kayvanpour E, Haas J, Lehmann DH, Gi WT, Frese KS, Nietsch R, Fehlmann T, Sandke S, Weis T, Keller A, Katus HA, Halle M, Frey N, Meder B, Sedaghat-Hamedani F (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Journal of Clinical Medicine MDPI

Book Volume: 11

Article Number: 5

Journal Issue: 1

DOI: 10.3390/jcm11010005

Abstract

Background: The current gold standard biomarker for myocardial infarction (MI), cardiac troponin (cTn), is recognized for its high sensitivity and organ specificity; however, it lacks diagnostic specificity. Numerous studies have introduced circulating microRNAs as potential bi-omarkers for MI. This study investigates the MI-specificity of these serum microRNAs by investi-gating myocardial stress/injury due to strenuous exercise. Methods: MicroRNA biomarkers were retrieved by comprehensive review of 109 publications on diagnostic serum microRNAs for MI. MicroRNA levels were first measured by next-generation sequencing in pooled sera from runners (n = 46) before and after conducting a full competitive marathon. Hereafter, reverse transcription quantitative real-time PCR (qPCR) of 10 selected serum microRNAs in 210 marathon runners was performed (>10,000 qPCR measurements). Results: 27 potential diagnostic microRNA for MI were retrieved by the literature review. Eight microRNAs (miR-1-3p, miR-21-5p, miR-26a-5p, miR-122-5p, miR-133a-3p, miR-142-5p, miR-191-5p, miR-486-3p) showed positive correlations with cTnT in marathon runners, whereas two miRNAs (miR-134-5p and miR-499a-5p) showed no correlations. Upregulation of miR-133a-3p (p = 0.03) and miR-142-5p (p = 0.01) went along with elevated cTnT after marathon. Conclusion: Some MI-associated microRNAs (e.g., miR-133a-3p and miR-142-5p) have similar kinetics under strenuous exercise and MI as compared to cTnT, which suggests that their diagnostic specificity could be limited. In contrast, several MI-associated microRNAs (miR-26a-5p, miR-134-5p, miR-191-5p) showed different release behavior; hence, combining cTnT with these microRNAs within a multi-marker strategy may add diagnostic accuracy in MI.

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Technische Universität München (TUM) DE Germany (DE) Universität des Saarlandes (UdS) DE Germany (DE) Deutsches Zentrum für Herz-Kreislauf-Forschung (DZHK e.V.) DE Germany (DE)

How to cite

APA:

Shirvani Samani, O., Scherr, J., Kayvanpour, E., Haas, J., Lehmann, D.H., Gi, W.-T.,... Sedaghat-Hamedani, F. (2022). Marathon-induced cardiac strain as model for the evaluation of diagnostic micrornas for acute myocardial infarction. Journal of Clinical Medicine, 11(1). https://doi.org/10.3390/jcm11010005

MLA:

Shirvani Samani, Omid, et al. "Marathon-induced cardiac strain as model for the evaluation of diagnostic micrornas for acute myocardial infarction." Journal of Clinical Medicine 11.1 (2022).

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