Antigen B modulates anti-inflammatory cytokines in the EAE model of multiple sclerosis
Mariki A, Barzin Z, Fasihi Harandi M, Karbasi Ravari K, Davoodi M, Mousavi SM, Rezakhani S, Nazeri Rezaabad M, Shabani M (2022)
Publication Type: Journal article
Publication year: 2022
Journal
DOI: 10.1002/brb3.2874
Abstract
Introduction: Multiple sclerosis (MS) is characterized by the destruction of the blood-brain barrier, loss of myelin sheath, and contribution of inflammatory interleukins such as TNF-alpha, interleukin-17, and interleukin-6. Methods: The current study investigated the effect of antigen B of hydatid cyst fluid on the reduction of anti-inflammatory cytokines and nerve conduction velocity in rats with experimental autoimmune encephalomyelitis (EAE)-induced MS. After isolation of antigen B from sterile cyst fluid, the rats were randomly divided into four groups: saline, EAE, EAE + teriflunomide (EAE + TF), and EAE + antigen B (EAE + AngB). The EAE model was induced using cow spinal cord homogenization, in combination with Freund's complete adjuvant. The serum concentration of cytokines including IL-1B and IL-17, IL-10, IL-6, and TNF-X was measured by the ELISA method, and real-time PCR was performed to study gene expression. Electrophysiological, behavioral, and neuropathological tests were also conducted. Results: Nerve conduction velocity and IL-10 concentration were increased in the antigen B group. The results of this study showed that antigen B reduced the inflammatory component of the EAE MS animal model by modulating the immune system compared to teriflunomide, which eventually led to a reduction in symptoms at the behavioral and electrophysiological level. Conclusions: It seems that antigen B plays a critical role in regulating immunity and it can be used as a possible therapeutic agent to modulate the immune system in MS patients. It might be rational to consider hydatid cyst fluid antigen as a modifier in MS.
Authors with CRIS profile
Involved external institutions
Jiroft University of Medical Sciences / دانشگاه علوم پزشکی و خدمات بهداشتی درمانی جیرفت
Iran, Islamic Republic of (IR)
Yasuj University of Medical Sciences (YUMS)
Iran, Islamic Republic of (IR)
Kerman University of Medical Sciences / Kerman Medical University
Iran, Islamic Republic of (IR)
Iran, Islamic Republic of (IR)
Yasuj University of Medical Sciences (YUMS)
Iran, Islamic Republic of (IR)
Kerman University of Medical Sciences / Kerman Medical University
Iran, Islamic Republic of (IR)
How to cite
APA:
Mariki, A., Barzin, Z., Fasihi Harandi, M., Karbasi Ravari, K., Davoodi, M., Mousavi, S.M.,... Shabani, M. (2022). Antigen B modulates anti-inflammatory cytokines in the EAE model of multiple sclerosis. Brain and Behavior. https://dx.doi.org/10.1002/brb3.2874
MLA:
Mariki, Aliakbar, et al. "Antigen B modulates anti-inflammatory cytokines in the EAE model of multiple sclerosis." Brain and Behavior (2022).
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