Kandathil AJ, Graw F, Quinn J, Hwang HS, Torbenson M, Perelson AS, Ray SC, Thomas DL, Ribeiro RM, Balagopal A (2013)
Publication Type: Journal article
Publication year: 2013
Book Volume: 145
Pages Range: 1404-1413.e10
Journal Issue: 6
DOI: 10.1053/j.gastro.2013.08.034
Background & Aims Hepatitis C virus (HCV) predominantly infects hepatocytes, but many hepatocytes are not infected; studies have shown that HCV antigens cluster within the liver. We investigated spatial distribution and determinants of HCV replication in human liver samples. Methods We analyzed liver samples from 4 patients with chronic HCV infection (genotype 1, Metavir scores 0-1) to estimate the proportion of infected hepatocytes and the amount of HCV viral RNA (vRNA) per cell. Single-cell laser capture microdissection was used to capture more than 1000 hepatocytes in grids, to preserve geometric relationships. HCV vRNA and interferon-induced transmembrane protein 3 (IFITM3) messenger RNA (the transcript of an interferon-stimulated gene) were measured in the same hepatocytes by quantitative polymerase chain reaction and assembled in maps to identify areas of high and low HCV replication. Results Patients' serum levels of HCV RNA ranged from 6.87 to 7.40 log
APA:
Kandathil, A.J., Graw, F., Quinn, J., Hwang, H.S., Torbenson, M., Perelson, A.S.,... Balagopal, A. (2013). Use of laser capture microdissection to map hepatitis C virus-positive hepatocytes in human liver. Gastroenterology, 145(6), 1404-1413.e10. https://doi.org/10.1053/j.gastro.2013.08.034
MLA:
Kandathil, Abraham J., et al. "Use of laser capture microdissection to map hepatitis C virus-positive hepatocytes in human liver." Gastroenterology 145.6 (2013): 1404-1413.e10.
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