Schneider Y, Turan S, Koller A, Krumbiegel M, Farrell M, Plotz S, Winkler J, Xiang W (2022)
Publication Type: Journal article
Publication year: 2022
Book Volume: 65
DOI: 10.1016/j.scr.2022.102952
Aggregation of alpha-synuclein (aSyn) is closely linked to Parkinson's disease, probably due to the loss of physiological functions and/or gain of toxic functions of aggregated aSyn. Significant efforts have been made elucidating the physiological structure and function of aSyn, however, with limited success thus far in human -derived cells, partly because of restricted resources. Here, we developed two human-induced pluripotent stem cell lines using CRISPR/Cas9-mediated allele-specific frame-shift deletion of the aSyn encoding gene SNCA, resulting in homo-and heterozygous SNCA knockout. The generated cell lines are promising cellular tools for studying aSyn dosage-dependent functions and structural alterations in human neural cells.
APA:
Schneider, Y., Turan, S., Koller, A., Krumbiegel, M., Farrell, M., Plotz, S.,... Xiang, W. (2022). Generation of a homozygous and a heterozygous SNCA gene knockout human-induced pluripotent stem cell line by CRISPR/Cas9 mediated allele-specific tuning of SNCA expression. Stem Cell Research, 65. https://doi.org/10.1016/j.scr.2022.102952
MLA:
Schneider, Yanni, et al. "Generation of a homozygous and a heterozygous SNCA gene knockout human-induced pluripotent stem cell line by CRISPR/Cas9 mediated allele-specific tuning of SNCA expression." Stem Cell Research 65 (2022).
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