Surfactant depletion combined with injurious ventilation results in a reproducible model of the acute respiratory distress syndrome (Ards)

Russ M, Boerger E, Von Platen P, Francis RCE, Taher M, Boemke W, Lachmann B, Leonhardt S, Pickerodt PA (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Journal of Visualized Experiments MYJoVE Corporation

Book Volume: 2021

Article Number: e62327

Journal Issue: 170

DOI: 10.3791/62327

Abstract

Various animal models exist to study the complex pathomechanisms of the acute respiratory distress syndrome (ARDS). These models include pulmo-arterial infusion of oleic acid, infusion of endotoxins or bacteria, cecal ligation and puncture, various pneumonia models, lung ischemia/reperfusion models and, of course, surfactant depletion models, among others. Surfactant depletion produces a rapid, reproducible deterioration of pulmonary gas exchange and hemodynamics and can be induced in anesthetized pigs using repeated lung lavages with 0.9% saline (35 mL/kg body weight, 37 °C). The surfactant depletion model supports investigations with standard respiratory and hemodynamic monitoring with clinically applied devices. But the model suffers from a relatively high recruitability and ventilation with high airway pressures can immediately reduce the severity of the injury by reopening atelectatic lung areas. Thus, this model is not suitable for investigations of ventilator regimes that use high airway pressures. A combination of surfactant depletion and injurious ventilation with high tidal volume/low positive end-expiratory pressure (high Tv/low PEEP) to cause ventilator induced lung injury (VILI) will reduce the recruitability of the resulting lung injury. The advantages of a timely induction and the possibility to perform experimental research in a setting comparable to an intensive care unit are preserved.

Authors with CRIS profile

Roland Francis

Involved external institutions

Charité - Universitätsmedizin Berlin DE Germany (DE) Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen DE Germany (DE)

How to cite

APA:

Russ, M., Boerger, E., Von Platen, P., Francis, R.C.E., Taher, M., Boemke, W.,... Pickerodt, P.A. (2021). Surfactant depletion combined with injurious ventilation results in a reproducible model of the acute respiratory distress syndrome (Ards). Journal of Visualized Experiments, 2021(170). https://doi.org/10.3791/62327

MLA:

Russ, Martin, et al. "Surfactant depletion combined with injurious ventilation results in a reproducible model of the acute respiratory distress syndrome (Ards)." Journal of Visualized Experiments 2021.170 (2021).

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