Intracellular ca2+ and not the extracellular matrix determines surface dynamics of AMPA-type glutamate receptors on aspiny neurons

Klueva J, Gundelfinger ED, Frischknecht RR, Heine M (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Philosophical Transactions of the Royal Society B-Biological Sciences Royal Society, The

Book Volume: 369

Journal Issue: 1654

DOI: 10.1098/rstb.2013.0605

Abstract

The perisynaptic extracellular matrix (ECM) contributes to the control of the lateral mobility of AMPA-type glutamate receptors (AMPARs) at spine synapses of principal hippocampal neurons. Here, we have studied the effect of the ECM on the lateral mobility of AMPARs at shaft synapses of aspiny interneurons. Single particle tracking experiments revealed that the removal of the hyaluronan-based ECM with hyaluronidase does not affect lateral receptor mobility on the timescale of seconds. Similarly, cross-linking with specific antibodies against the extracellular domain of the GluA1 receptor subunit, which affects lateral receptor mobility on spiny neurons, does not influence receptor mobility on aspiny neurons. AMPARs on aspiny inter-neurons are characterized by strong inward rectification indicating a significant fraction of Ca2+-permeable receptors. Therefore, we tested whether Ca2+ controls AMPAR mobility in these neurons. Application of the membrane-permeable Ca2+ chelator BAPTA-AM significantly increased the lateral mobility of GluA1-containing synaptic and extrasynaptic receptors. These data indicate that the perisynaptic ECM affects the lateral mobility differently on spiny and aspiny neurons. Although ECM structures on inter-neurons appear much more prominent, their influence on AMPAR mobility seems to be negligible at short timescales.

Involved external institutions

Max-Planck-Institut für Neurobiologie / Max Planck Institute of Neurobiology DE Germany (DE)

How to cite

APA:

Klueva, J., Gundelfinger, E.D., Frischknecht, R.R., & Heine, M. (2014). Intracellular ca2+ and not the extracellular matrix determines surface dynamics of AMPA-type glutamate receptors on aspiny neurons. Philosophical Transactions of the Royal Society B-Biological Sciences, 369(1654). https://doi.org/10.1098/rstb.2013.0605

MLA:

Klueva, Julia, et al. "Intracellular ca2+ and not the extracellular matrix determines surface dynamics of AMPA-type glutamate receptors on aspiny neurons." Philosophical Transactions of the Royal Society B-Biological Sciences 369.1654 (2014).

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