The centrosome protein AKNA regulates neurogenesis via microtubule organization
Ortega GC, Falk S, Johansson PA, Peyre E, Broix L, Sahu SK, Hirst W, Schlichthaerle T, Romero CDJ, Draganova K, Vinopal S, Chinnappa K, Gavranovic A, Karakaya T, Steininger T, Merl-Pham J, Feederle R, Shao W, Shi SH, Hauck SM, Jungmann R, Bradke F, Borrell V, Geerlof A, Reber S, Tiwari VK, Huttner WB, Wilsch-Brauninger M, Nguyen L, Goetz M (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 567
Pages Range: 113-117
Journal Issue: 7746
DOI: 10.1038/s41586-019-0962-4
Abstract
The expansion of brain size is accompanied by a relative enlargement of the subventricular zone during development. Epithelial-like neural stem cells divide in the ventricular zone at the ventricles of the embryonic brain, self-renew and generate basal progenitors1 that delaminate and settle in the subventricular zone in enlarged brain regions2. The length of time that cells stay in the subventricular zone is essential for controlling further amplification and fate determination. Here we show that the interphase centrosome protein AKNA has a key role in this process. AKNA localizes at the subdistal appendages of the mother centriole in specific subtypes of neural stem cells, and in almost all basal progenitors. This protein is necessary and sufficient to organize centrosomal microtubules, and promote their nucleation and growth. These features of AKNA are important for mediating the delamination process in the formation of the subventricular zone. Moreover, AKNA regulates the exit from the subventricular zone, which reveals the pivotal role of centrosomal microtubule organization in enabling cells to both enter and remain in the subventricular zone. The epithelial-to-mesenchymal transition is also regulated by AKNA in other epithelial cells, demonstrating its general importance for the control of cell delamination.
Involved external institutions
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
University of Liège (ULg) / Université de Liège
Belgium (BE)
Institut für Molekulare Biologie gGmbH / Institute of Molecular Biology (IMB)
Germany (DE)
Humboldt-Universität zu Berlin
Germany (DE)
Max-Planck-Institut für Biochemie / Max Planck Institute of Biochemistry
Germany (DE)
Spanish National Research Council / Consejo Superior de Investigaciones Científicas (CSIC)
Spain (ES)
Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Germany (DE)
Memorial Sloan Kettering Cancer Center
United States (USA) (US)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Germany (DE)
University of Liège (ULg) / Université de Liège
Belgium (BE)
Institut für Molekulare Biologie gGmbH / Institute of Molecular Biology (IMB)
Germany (DE)
Humboldt-Universität zu Berlin
Germany (DE)
Max-Planck-Institut für Biochemie / Max Planck Institute of Biochemistry
Germany (DE)
Spanish National Research Council / Consejo Superior de Investigaciones Científicas (CSIC)
Spain (ES)
Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Germany (DE)
Memorial Sloan Kettering Cancer Center
United States (USA) (US)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
How to cite
APA:
Ortega, G.C., Falk, S., Johansson, P.A., Peyre, E., Broix, L., Sahu, S.K.,... Goetz, M. (2019). The centrosome protein AKNA regulates neurogenesis via microtubule organization. Nature, 567(7746), 113-117. https://doi.org/10.1038/s41586-019-0962-4
MLA:
Ortega, German Camargo, et al. "The centrosome protein AKNA regulates neurogenesis via microtubule organization." Nature 567.7746 (2019): 113-117.
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