Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding
Falk S, Bugeon S, Ninkovic J, Pilz GA, Postiglione MP, Cremer H, Knoblich JA, Goetz M (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 93
Pages Range: 777-791.e3
Journal Issue: 4
DOI: 10.1016/j.neuron.2017.02.009
Abstract
The developmental mechanisms regulating the number of adult neural stem cells (aNSCs) are largely unknown. Here we show that the cleavage plane orientation in murine embryonic radial glia cells (RGCs) regulates the number of aNSCs in the lateral ganglionic eminence (LGE). Randomizing spindle orientation in RGCs by overexpression of Insc or a dominant-negative form of Lgn (dnLgn) reduces the frequency of self-renewing asymmetric divisions while favoring symmetric divisions generating two SNPs. Importantly, these changes during embryonic development result in reduced seeding of aNSCs. Interestingly, no effects on aNSC numbers were observed when Insc was overexpressed in postnatal RGCs or aNSCs. These data suggest a new mechanism for controlling aNSC numbers and show that the role of spindle orientation during brain development is highly time and region dependent.
Involved external institutions
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Aix-Marseille University / Aix-Marseille Université
France (FR)
Institut für Molekulare Biotechnologie / Institute of Molecular Biotechnology (IMBA)
Austria (AT)
Germany (DE)
Aix-Marseille University / Aix-Marseille Université
France (FR)
Institut für Molekulare Biotechnologie / Institute of Molecular Biotechnology (IMBA)
Austria (AT)
How to cite
APA:
Falk, S., Bugeon, S., Ninkovic, J., Pilz, G.-A., Postiglione, M.P., Cremer, H.,... Goetz, M. (2017). Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding. Neuron, 93(4), 777-791.e3. https://doi.org/10.1016/j.neuron.2017.02.009
MLA:
Falk, Sven, et al. "Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding." Neuron 93.4 (2017): 777-791.e3.
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