Niebler S, Bosserhoff AK (2013)
Publication Type: Journal article
Publication year: 2013
Book Volume: 280
Pages Range: 1397-1408
Journal Issue: 6
DOI: 10.1111/febs.12130
The transcription factor activating enhancer-binding protein epsilon (AP-2ε) was recently shown to be expressed during late chondrocyte differentiation, especially in hypertrophic chondrocytes. In this study, we were able to reveal that the promoter of the type II collagen (COL2A1) gene, encoding the extracellular matrix protein type II collagen, is specifically regulated by AP-2ε. Expression of COL2A1 is downregulated at the transition of chondroblasts into hypertrophic chondrocytes and our data provide evidence that AP-2ε is involved in this process. In reporter gene assays, overexpression of AP-2ε in cartilaginous cell lines resulted in a significant reduction in COL2A1 core promoter activity of ~ 45%. Furthermore, we found that this process is dose-dependent and highly specific for the epsilon isoform. Computational analysis offered only a single putative AP-2-binding motif within the chosen promoter fragment but site-directed mutagenesis revealed this motif to be regulatory inactive. After expanding our screening to motifs containing minor differences from the classical AP-2 consensus sequence (5′-GCCN
APA:
Niebler, S., & Bosserhoff, A.K. (2013). The transcription factor activating enhancer-binding protein epsilon (AP-2ε) regulates the core promoter of type II collagen (COL2A1). Febs Journal, 280(6), 1397-1408. https://doi.org/10.1111/febs.12130
MLA:
Niebler, Stephan, and Anja Katrin Bosserhoff. "The transcription factor activating enhancer-binding protein epsilon (AP-2ε) regulates the core promoter of type II collagen (COL2A1)." Febs Journal 280.6 (2013): 1397-1408.
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