Transfection Methods
Ruedel A, Bosserhoff AK (2012)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2012
Journal
Edited Volumes: Transfection Methods
Book Volume: 112
Pages Range: 163-182
DOI: 10.1016/B978-0-12-405914-6.00008-1
Abstract
It is an important method in molecular biology to up- or downregulate gene expression in cells. By transfection with expression constructs or silencing RNAs, the functionon genes can be defined and cellular reactions can be analyzed. For this purpose several methods are known and used to date. This article illustrates the most common protocols to introduce DNA or RNA into cells, including lipofection and electroporation. In the case of lipofection the nucleic acid is encapsulated in liposomes which fuse with the cell membrane, thereby releasing the nucleic acid into the cell. Cell division is required for DNA to get into the nucleus to be transcribed. In contrast to that, electroporation utilizes electrical pulses to create small pores in the cell membrane. This method allows transfection directly into the nucleus and therefore does not require cell division. Taken together, all methods can be used for cell transfection but it always has to be tested experimentally which method works best for each cell type. © 2012, Elsevier Inc. All rights reserved.
Involved external institutions
Germany (DE)How to cite
APA:
Ruedel, A., & Bosserhoff, A.K. (2012). Transfection Methods. In Methods in Cell Biology (Eds.), Transfection Methods. (pp. 163-182).
MLA:
Ruedel, A., and A. K. Bosserhoff. "Transfection Methods." Transfection Methods. Ed. Methods in Cell Biology, 2012. 163-182.
BibTeX: Download