A bilayered tissue engineered in vitro model simulating the tooth periodontium
Khadre A, Raif EL, Junaid S, Goudouri OM, Refaat W, Ramadan A, Boccaccini AR, El-Gendy R (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 42
Pages Range: 232-245
DOI: 10.22203/eCM.v042a17
Abstract
Due to the complexity of the structure of the tooth periodontium, regeneration of the full tooth attachment is not a trivial task. There is also a gap in models that can represent human tooth attachment in vitro and in vivo. The aim of this study was to develop a bilayered in vitro construct that simulated the tooth periodontal ligament and attached alveolar bone, for the purpose of tissue regeneration and investigation of physiological and orthodontic loading. Two types of materials were used to develop this construct: sol-gel 60S10Mg derived scaffold, representing the hard tissue component of the periodontium, and commercially available Geistlich Bio-Gide® collagen membrane, representing the soft tissue component of the tooth attachment. Each scaffold was dynamically seeded with human periodontal ligament cells (HPDLCs). Scaffolds were either cultured separately, or combined in a bilayered construct, for 2 weeks. Characterisation of the individual scaffolds and the bilayered constructs included biological characterisation (cell viability, scanning electron microscopy to confirm cell attachment, gene expression of periodontium regeneration markers), and mechanical characterisation of scaffolds and constructs. HPDLCs enjoyed a biocompatible 3-dimensional environment within the bilayered construct components. There was no drop in cellular gene expression in the bilayered construct, compared to the separate scaffolds.
Involved external institutions
United Kingdom (GB)How to cite
APA:
Khadre, A., Raif, E.L., Junaid, S., Goudouri, O.M., Refaat, W., Ramadan, A.,... El-Gendy, R. (2021). A bilayered tissue engineered in vitro model simulating the tooth periodontium. European Cells & Materials, 42, 232-245. https://dx.doi.org/10.22203/eCM.v042a17
MLA:
Khadre, A., et al. "A bilayered tissue engineered in vitro model simulating the tooth periodontium." European Cells & Materials 42 (2021): 232-245.
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