Blockade of tumour necrosis factor-α in experimental autoimmune encephalomyelitis reveals differential effects on the antigen-specific immune response and central nervous system histopathology
Batoulis H, Recks MS, Holland FO, Thomalla F, Williams RO, Kuerten S (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Book Volume: 175
Pages Range: 41-48
Journal Issue: 1
DOI: 10.1111/cei.12209
Abstract
In various autoimmune diseases, anti-tumour necrosis factor (TNF)-α treatment has been shown to reduce both clinical disease severity and T helper type 1 (Th1)1/Th17 responses. In experimental autoimmune encephalomyelitis (EAE), however, the role of TNF-α has remained unclear. Here, C57BL/6 mice were immunized with myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 and treated with anti-TNF-α, control antibody or vehicle. The clinical disease course, incidence and severity were assessed. On day 20 after immunization the antigen-specific Th1/Th17 response was evaluated by enzyme-linked immunospot (ELISPOT) in spleen and central nervous system (CNS). Also, the extent of spinal cord histopathology was analysed on semi- and ultrathin sections. Our results demonstrate that anti-TNF-α treatment reduced the incidence and delayed the onset of EAE, but had no effect on disease severity once EAE had been established. Whereas anti-TNF-α treatment induced an increase in splenic Th1/Th17 responses, there was no effect on the number of antigen-specific Th1/Th17 cells in the spinal cord. Accordingly, the degree of CNS histopathology was comparable in control and anti-TNF-α-treated mice. In conclusion, while the anti-TNF-α treatment had neither immunosuppressive effects on the Th1/Th17 response in the CNS nor histoprotective properties in EAE, it enhanced the myelin-specific T cell response in the immune periphery. © 2013 British Society for Immunology.
Authors with CRIS profile
Involved external institutions
Universität zu Köln
Germany (DE)
University of Oxford
United Kingdom (GB)
Julius-Maximilians-Universität Würzburg
Germany (DE)
Germany (DE)
University of Oxford
United Kingdom (GB)
Julius-Maximilians-Universität Würzburg
Germany (DE)
How to cite
APA:
Batoulis, H., Recks, M.S., Holland, F.O., Thomalla, F., Williams, R.O., & Kuerten, S. (2014). Blockade of tumour necrosis factor-α in experimental autoimmune encephalomyelitis reveals differential effects on the antigen-specific immune response and central nervous system histopathology. Clinical and Experimental Immunology, 175(1), 41-48. https://doi.org/10.1111/cei.12209
MLA:
Batoulis, H., et al. "Blockade of tumour necrosis factor-α in experimental autoimmune encephalomyelitis reveals differential effects on the antigen-specific immune response and central nervous system histopathology." Clinical and Experimental Immunology 175.1 (2014): 41-48.
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