Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium
Reichel F, Kraeter M, Peikert K, Glass H, Rosendahl P, Herbig M, Prieto AR, Kihm A, Bosman G, Kaestner L, Hermann A, Guck J (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 13
Article Number: 852946
DOI: 10.3389/fphys.2022.852946
Abstract
Misshaped red blood cells (RBCs), characterized by thorn-like protrusions known as acanthocytes, are a key diagnostic feature in Chorea-Acanthocytosis (ChAc), a rare neurodegenerative disorder. The altered RBC morphology likely influences their biomechanical properties which are crucial for the cells to pass the microvasculature. Here, we investigated blood cell deformability of five ChAc patients compared to healthy controls during up to 1-year individual off-label treatment with the tyrosine kinase inhibitor dasatinib or several weeks with lithium. Measurements with two microfluidic techniques allowed us to assess RBC deformability under different shear stresses. Furthermore, we characterized leukocyte stiffness at high shear stresses. The results showed that blood cell deformability–including both RBCs and leukocytes - in general was altered in ChAc patients compared to healthy donors. Therefore, this study shows for the first time an impairment of leukocyte properties in ChAc. During treatment with dasatinib or lithium, we observed alterations in RBC deformability and a stiffness increase for leukocytes. The hematological phenotype of ChAc patients hinted at a reorganization of the cytoskeleton in blood cells which partly explains the altered mechanical properties observed here. These findings highlight the need for a systematic assessment of the contribution of impaired blood cell mechanics to the clinical manifestation of ChAc.
Authors with CRIS profile
Involved external institutions
Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light
Germany (DE)
Universität Rostock
Germany (DE)
Technische Universität Dresden
Germany (DE)
Universität des Saarlandes (UdS)
Germany (DE)
Radboud University Nijmegen Medical Centre / Radboudumc of voluit Radboud Universitair Medisch Centrum (UMC)
Netherlands (NL)
Germany (DE)
Universität Rostock
Germany (DE)
Technische Universität Dresden
Germany (DE)
Universität des Saarlandes (UdS)
Germany (DE)
Radboud University Nijmegen Medical Centre / Radboudumc of voluit Radboud Universitair Medisch Centrum (UMC)
Netherlands (NL)
How to cite
APA:
Reichel, F., Kraeter, M., Peikert, K., Glass, H., Rosendahl, P., Herbig, M.,... Guck, J. (2022). Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium. Frontiers in Physiology, 13. https://doi.org/10.3389/fphys.2022.852946
MLA:
Reichel, Felix, et al. "Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium." Frontiers in Physiology 13 (2022).
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