Intelligent image-based deformation-assisted cell sorting with molecular specificity
Nawaz AA, Urbanska M, Herbig M, Noetzel M, Kraeter M, Rosendahl P, Herold C, Toepfner N, Kubankova M, Goswami R, Abuhattum S, Reichel F, Mueller P, Taubenberger A, Girardo S, Jacobi A, Guck J (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 17
Pages Range: 595-599
Journal Issue: 6
DOI: 10.1038/s41592-020-0831-y
Abstract
Although label-free cell sorting is desirable for providing pristine cells for further analysis or use, current approaches lack molecular specificity and speed. Here, we combine real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves and transfer molecular specificity to image-based sorting using an efficient deep neural network. In addition to general performance, we demonstrate the utility of this method by sorting neutrophils from whole blood without labels.
Authors with CRIS profile
Involved external institutions
Technische Universität Dresden
Germany (DE)
ZELLMECHANIK DRESDEN GmbH
Germany (DE)
Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light
Germany (DE)
Germany (DE)
ZELLMECHANIK DRESDEN GmbH
Germany (DE)
Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light
Germany (DE)
How to cite
APA:
Nawaz, A.A., Urbanska, M., Herbig, M., Noetzel, M., Kraeter, M., Rosendahl, P.,... Guck, J. (2020). Intelligent image-based deformation-assisted cell sorting with molecular specificity. Nature Methods, 17(6), 595-599. https://doi.org/10.1038/s41592-020-0831-y
MLA:
Nawaz, Ahmad Ahsan, et al. "Intelligent image-based deformation-assisted cell sorting with molecular specificity." Nature Methods 17.6 (2020): 595-599.
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