Recent progress and current opinions in Brillouin microscopy for life science applications
Antonacci G, Beck T, Bilenca A, Czarske J, Elsayad K, Kim K, Krug B, Palombo F, Prevedel R, Scarcelli G, Guck J (2020)
Publication Type: Journal article, Review article
Publication year: 2020
Journal
Book Volume: 12
Pages Range: 615-624
Journal Issue: 3
DOI: 10.1007/s12551-020-00701-9
Abstract
Many important biological functions and processes are reflected in cell and tissue mechanical properties such as elasticity and viscosity. However, current techniques used for measuring these properties have major limitations, such as that they can often not measure inside intact cells and/or require physical contact—which cells can react to and change. Brillouin light scattering offers the ability to measure mechanical properties in a non-contact and label-free manner inside of objects with high spatial resolution using light, and hence has emerged as an attractive method during the past decade. This new approach, coined “Brillouin microscopy,” which integrates highly interdisciplinary concepts from physics, engineering, and mechanobiology, has led to a vibrant new community that has organized itself via a European funded (COST Action) network. Here we share our current assessment and opinion of the field, as emerged from a recent dedicated workshop. In particular, we discuss the prospects towards improved and more bio-compatible instrumentation, novel strategies to infer more accurate and quantitative mechanical measurements, as well as our current view on the biomechanical interpretation of the Brillouin spectra.
Authors with CRIS profile
Involved external institutions
Universiteit Gent (UGent) / Ghent University
Belgium (BE)
Technische Universität Dresden
Germany (DE)
Ben-Gurion University of the Negev (BGU) / אוניברסיטת בן-גוריון בנגב
Israel (IL)
Vienna Biocenter VBC
Austria (AT)
University of Exeter
United Kingdom (GB)
European Molecular Biology Laboratory (EMBL)
Germany (DE)
University of Maryland
United States (USA) (US)
Belgium (BE)
Technische Universität Dresden
Germany (DE)
Ben-Gurion University of the Negev (BGU) / אוניברסיטת בן-גוריון בנגב
Israel (IL)
Vienna Biocenter VBC
Austria (AT)
University of Exeter
United Kingdom (GB)
European Molecular Biology Laboratory (EMBL)
Germany (DE)
University of Maryland
United States (USA) (US)
How to cite
APA:
Antonacci, G., Beck, T., Bilenca, A., Czarske, J., Elsayad, K., Kim, K.,... Guck, J. (2020). Recent progress and current opinions in Brillouin microscopy for life science applications. Biophysical Reviews, 12(3), 615-624. https://doi.org/10.1007/s12551-020-00701-9
MLA:
Antonacci, Giuseppe, et al. "Recent progress and current opinions in Brillouin microscopy for life science applications." Biophysical Reviews 12.3 (2020): 615-624.
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