Real-time deformability cytometry: Label-free functional characterization of cells
Herbig M, Kraeter M, Plak K, Mueller P, Guck J, Otto O (2018)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2018
Journal
Publisher: Humana Press Inc.
Series: Methods in Molecular Biology
Book Volume: 1678
Pages Range: 347-369
DOI: 10.1007/978-1-4939-7346-0_15
Abstract
Real-time deformability cytometry (RT-DC) is a microfluidic technique that allows to capture and evaluate morphology and rheology of up to 1000 cells/s in a constricted channel. The cells are deformed without mechanical contact by hydrodynamic forces and are quantified in real-time without the need of additional handling or staining procedures. Segmented pictures of the cells are stored and can be used for further analysis. RT-DC is sensitive to alterations of the cytoskeleton, which allows, e.g., to show differences in cell cycle phases, identify different subpopulations in whole blood and to study mechanical stiffening of cells entering a dormant state. The abundance of the obtainable parameters and the interpretation as mechanical readout is an analytical challenge that needs standardization. Here, we will provide guidelines for measuring and post-processing of RT-DC data.
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Germany (DE)How to cite
APA:
Herbig, M., Kraeter, M., Plak, K., Mueller, P., Guck, J., & Otto, O. (2018). Real-time deformability cytometry: Label-free functional characterization of cells. In (pp. 347-369). Humana Press Inc..
MLA:
Herbig, Maik, et al. "Real-time deformability cytometry: Label-free functional characterization of cells." Humana Press Inc., 2018. 347-369.
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