Three-dimensional correlative single-cell imaging utilizing fluorescence and refractive index tomography
Schuermann M, Cojoc G, Girardo S, Ulbricht E, Guck J, Mueller P (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 11
Article Number: e201700145
Journal Issue: 3
Abstract
Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI). ODT is complementary to fluorescence imaging and does not require any markers. The present study introduces RI and fluorescence tomography with optofluidic rotation (RAFTOR) of suspended cells, facilitating the segmentation of the 3D-correlated RI and fluorescence data for a quantitative interpretation of the nuclear RI. The technique is validated with cell phantoms and used to confirm a lower nuclear RI for HL60 cells. Furthermore, the nuclear inversion of adult mouse photoreceptor cells is observed in the RI distribution. The applications shown confirm predictions of previous studies and illustrate the potential of RAFTOR to improve our understanding of cells and tissues.
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Germany (DE)How to cite
APA:
Schuermann, M., Cojoc, G., Girardo, S., Ulbricht, E., Guck, J., & Mueller, P. (2018). Three-dimensional correlative single-cell imaging utilizing fluorescence and refractive index tomography. Journal of Biophotonics, 11(3). https://doi.org/10.1002/jbio.201700145
MLA:
Schuermann, Mirjam, et al. "Three-dimensional correlative single-cell imaging utilizing fluorescence and refractive index tomography." Journal of Biophotonics 11.3 (2018).
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