Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging
Mueller P, Schuermann M, Girardo S, Cojoc G, Guck J (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 26
Pages Range: 10729-10743
Journal Issue: 8
DOI: 10.1364/OE.26.010729
Abstract
Measuring the average refractive index (RI) of spherical objects, such as suspended cells, in quantitative phase imaging (QPI) requires a decoupling of RI and size from the QPI data. This has been commonly achieved by determining the object’s radius with geometrical approaches, neglecting light-scattering. Here, we present a novel QPI fitting algorithm that reliably uncouples the RI using Mie theory and a semi-analytical, corrected Rytov approach. We assess the range of validity of this algorithm in silico and experimentally investigate various objects (oil and protein droplets, microgel beads, cells) and noise conditions. In addition, we provide important practical cues for the analysis of spherical objects in QPI.
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Germany (DE)How to cite
APA:
Mueller, P., Schuermann, M., Girardo, S., Cojoc, G., & Guck, J. (2018). Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging. Optics Express, 26(8), 10729-10743. https://doi.org/10.1364/OE.26.010729
MLA:
Mueller, Paul, et al. "Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging." Optics Express 26.8 (2018): 10729-10743.
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