Real-time fluorescence and deformability cytometry

Rosendahl P, Plak K, Jacobi A, Kraeter M, Toepfner N, Otto O, Herold C, Winzi M, Herbig M, Ge Y, Girardo S, Wagner K, Baum B, Guck J (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Nature Methods Nature Publishing Group

Book Volume: 15

Pages Range: 355-358

Journal Issue: 5

DOI: 10.1038/nmeth.4639

Abstract

The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.

Authors with CRIS profile

Jochen Guck

Involved external institutions

Technische Universität Dresden DE Germany (DE) University College London (UCL) GB United Kingdom (GB)

How to cite

APA:

Rosendahl, P., Plak, K., Jacobi, A., Kraeter, M., Toepfner, N., Otto, O.,... Guck, J. (2018). Real-time fluorescence and deformability cytometry. Nature Methods, 15(5), 355-358. https://doi.org/10.1038/nmeth.4639

MLA:

Rosendahl, Philipp, et al. "Real-time fluorescence and deformability cytometry." Nature Methods 15.5 (2018): 355-358.

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