Porter L, Toepfner N, Bashant KR, Guck J, Ashcroft M, Farahi N, Chilvers ER (2018)
Publication Type: Journal article
Publication year: 2018
Book Volume: 9
Article Number: 1404
Journal Issue: JUN
Immune cells face constant changes in their microenvironment, which requires rapid metabolic adaptation. In contrast to neutrophils, which are known to rely near exclusively on glycolysis, the metabolic profile of human eosinophils has not been characterized. Here, we assess the key metabolic parameters of peripheral blood-derived human eosinophils using real-time extracellular flux analysis to measure extracellular acidification rate and oxygen consumption rate, and compare these parameters to human neutrophils. Using this methodology, we demonstrate that eosinophils and neutrophils have a similar glycolytic capacity, albeit with a minimal glycolytic reserve. However, compared to neutrophils, eosinophils exhibit significantly greater basal mitochondrial respiration, ATP-linked respiration, maximum respiratory capacity, and spare respiratory capacity. Of note, the glucose oxidation pathway is also utilized by eosinophils, something not evident in neutrophils. Furthermore, using a colorimetric enzymatic assay, we show that eosinophils have much reduced glycogen stores compared to neutrophils. We also show that physiologically relevant levels of hypoxia (PO
APA:
Porter, L., Toepfner, N., Bashant, K.R., Guck, J., Ashcroft, M., Farahi, N., & Chilvers, E.R. (2018). Metabolic profiling of human eosinophils. Frontiers in Immunology, 9(JUN). https://doi.org/10.3389/fimmu.2018.01404
MLA:
Porter, Linsey, et al. "Metabolic profiling of human eosinophils." Frontiers in Immunology 9.JUN (2018).
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