Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis
Regnault C, Punyani K, Holm S, Beech JP, Otto O, Herold C, Herbig M, Tegenfeldt JO, Barrett MP, Guck J (2016)
Publication Type: Conference contribution
Publication year: 2016
Publisher: Chemical and Biological Microsystems Society
Pages Range: 244-245
Conference Proceedings Title: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Event location: Dublin
ISBN: 9780979806490
Abstract
Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.
Authors with CRIS profile
Involved external institutions
University of Glasgow
United Kingdom (GB)
Lund University / Lunds universitet
Sweden (SE)
Technische Universität Dresden
Germany (DE)
United Kingdom (GB)
Lund University / Lunds universitet
Sweden (SE)
Technische Universität Dresden
Germany (DE)
How to cite
APA:
Regnault, C., Punyani, K., Holm, S., Beech, J.P., Otto, O., Herold, C.,... Guck, J. (2016). Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 244-245). Dublin, IE: Chemical and Biological Microsystems Society.
MLA:
Regnault, C., et al. "Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis." Proceedings of the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin Chemical and Biological Microsystems Society, 2016. 244-245.
BibTeX: Download