Real-time deformability cytometry: On-the-fly cell mechanical phenotyping
Otto O, Rosendahl P, Mietke A, Golfier S, Herold C, Klaue D, Girardo S, Pagliara S, Ekpenyong A, Jacobi A, Wobus M, Toepfner N, Keyser UF, Mansfeld J, Fischer-Friedrich E, Guck J (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 12
Pages Range: 199-202
Journal Issue: 3
DOI: 10.1038/nmeth.3281
Abstract
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
Authors with CRIS profile
Involved external institutions
Technische Universität Dresden
Germany (DE)
University of Cambridge
United Kingdom (GB)
Max-Planck-Institut für Physik komplexer Systeme (MPI PKS) / Max Planck Institute for the Physics of Complex Systems
Germany (DE)
Germany (DE)
University of Cambridge
United Kingdom (GB)
Max-Planck-Institut für Physik komplexer Systeme (MPI PKS) / Max Planck Institute for the Physics of Complex Systems
Germany (DE)
How to cite
APA:
Otto, O., Rosendahl, P., Mietke, A., Golfier, S., Herold, C., Klaue, D.,... Guck, J. (2015). Real-time deformability cytometry: On-the-fly cell mechanical phenotyping. Nature methods, 12(3), 199-202. https://doi.org/10.1038/nmeth.3281
MLA:
Otto, Oliver, et al. "Real-time deformability cytometry: On-the-fly cell mechanical phenotyping." Nature methods 12.3 (2015): 199-202.
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