Deformation of phospholipid vesicles in an optical stretcher
Delabre U, Feld K, Crespo E, Whyte G, Sykes C, Seifert U, Guck J (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 11
Pages Range: 6075-6088
Journal Issue: 30
DOI: 10.1039/c5sm00562k
Abstract
Phospholipid vesicles are common model systems for cell membranes. Important aspects of the membrane function relate to its mechanical properties. Here we have investigated the deformation behaviour of phospholipid vesicles in a dual-beam laser trap, also called an optical stretcher. This study explicitly makes use of the inherent heating present in such traps to investigate the dependence of vesicle deformation on temperature. By using lasers with different wavelengths, optically induced mechanical stresses and temperature increase can be tuned fairly independently with a single setup. The phase transition temperature of vesicles can be clearly identified by an increase in deformation. In the case of no heating effects, a minimal model for drop deformation in an optical stretcher and a more specific model for vesicle deformation that takes explicitly into account the angular dependence of the optical stress are presented to account for the experimental results. Elastic constants are extracted from the fitting procedures, which agree with literature data. This study demonstrates the utility of optical stretching, which is easily combined with microfluidic delivery, for the future serial, high-throughput study of the mechanical and thermodynamic properties of phospholipid vesicles.
Authors with CRIS profile
Involved external institutions
Institut national de physique nucléaire et de physique des particules (IN2P3)
France (FR)
University of Cambridge
United Kingdom (GB)
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
Universität Stuttgart
Germany (DE)
France (FR)
University of Cambridge
United Kingdom (GB)
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
Universität Stuttgart
Germany (DE)
How to cite
APA:
Delabre, U., Feld, K., Crespo, E., Whyte, G., Sykes, C., Seifert, U., & Guck, J. (2015). Deformation of phospholipid vesicles in an optical stretcher. Soft Matter, 11(30), 6075-6088. https://doi.org/10.1039/c5sm00562k
MLA:
Delabre, Ulysse, et al. "Deformation of phospholipid vesicles in an optical stretcher." Soft Matter 11.30 (2015): 6075-6088.
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