Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
Mietke A, Otto O, Girardo S, Rosendahl P, Taubenberger A, Golfier S, Ulbricht E, Aland S, Guck J, Fischer-Friedrich E (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 109
Pages Range: 2023-2036
Journal Issue: 10
DOI: 10.1016/j.bpj.2015.09.006
Abstract
Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications in biology and medicine. A new method, real-time deformability cytometry, probes cell stiffness at high throughput by exposing cells to a shear flow in a microfluidic channel, allowing for mechanical phenotyping based on single-cell deformability. However, observed deformations of cells in the channel not only are determined by cell stiffness, but also depend on cell size relative to channel size. Here, we disentangle mutual contributions of cell size and cell stiffness to cell deformation by a theoretical analysis in terms of hydrodynamics and linear elasticity theory. Performing real-time deformability cytometry experiments on both model spheres of known elasticity and biological cells, we demonstrate that our analytical model not only predicts deformed shapes inside the channel but also allows for quantification of cell mechanical parameters. Thereby, fast and quantitative mechanical sampling of large cell populations becomes feasible.
Authors with CRIS profile
Involved external institutions
Technische Universität Dresden
Germany (DE)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Germany (DE)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
How to cite
APA:
Mietke, A., Otto, O., Girardo, S., Rosendahl, P., Taubenberger, A., Golfier, S.,... Fischer-Friedrich, E. (2015). Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment. Biophysical Journal, 109(10), 2023-2036. https://doi.org/10.1016/j.bpj.2015.09.006
MLA:
Mietke, Alexander, et al. "Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment." Biophysical Journal 109.10 (2015): 2023-2036.
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