Inflammation-induced brain endothelial activation leads to uptake of electrostatically stabilized iron oxide nanoparticles via sulfated glycosaminoglycans

Berndt D, Millward JM, Schnorr J, Taupitz M, Stangl V, Paul F, Wagner S, Wuerfel JT, Ludwig A, Infante-Duarte C, Sack I (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Nanomedicine-Nanotechnology Biology and Medicine Elsevier

Book Volume: 13

Pages Range: 1411-1421

Journal Issue: 4

DOI: 10.1016/j.nano.2017.01.010

Abstract

Based on our previous data on the presence of very small superparamagnetic iron oxide nanoparticles (VSOP) on brain endothelial structures during experimental autoimmune encephalomyelitis (EAE), we investigated the mechanisms of VSOP binding on inflamed brain endothelial cells in vivo and in vitro. After intravenous application, VSOP were detected in brain endothelial cells of EAE animals at peak disease and prior to clinical onset. In vitro, inflammatory stimuli increased VSOP uptake by brain endothelial bEnd.3 cells, which we confirmed in primary endothelial cells and in bEnd.3 cells cultured under shear stress. Transmission electron microscopy and blocking experiments revealed that during inflammation VSOP were endocytosed by bEnd.3. Modified sulfated glycosaminoglycans (GAG) on inflamed brain endothelial cells were the primary binding site for VSOP, as GAG degradation and inhibition of GAG sulfation reduced VSOP uptake. Thus, VSOP-based MRI is sensitive to visualize early neuroinflammatory processes such as GAG modifications on brain endothelial cells.

Involved external institutions

Charité - Universitätsmedizin Berlin DE Germany (DE) Max-Delbrück-Centrum für Molekulare Medizin / Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch DE Germany (DE)

How to cite

APA:

Berndt, D., Millward, J.M., Schnorr, J., Taupitz, M., Stangl, V., Paul, F.,... Sack, I. (2017). Inflammation-induced brain endothelial activation leads to uptake of electrostatically stabilized iron oxide nanoparticles via sulfated glycosaminoglycans. Nanomedicine-Nanotechnology Biology and Medicine, 13(4), 1411-1421. https://doi.org/10.1016/j.nano.2017.01.010

MLA:

Berndt, Dominique, et al. "Inflammation-induced brain endothelial activation leads to uptake of electrostatically stabilized iron oxide nanoparticles via sulfated glycosaminoglycans." Nanomedicine-Nanotechnology Biology and Medicine 13.4 (2017): 1411-1421.

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