Use of the TetOn system to study molecular mechanisms of zebrafish regeneration
Wehner D, Jahn C, Weidinger G (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 2015
Article Number: e52756
Journal Issue: 100
DOI: 10.3791/52756
Abstract
The zebrafish has become a very important model organism for studying vertebrate development, physiology, disease, and tissue regeneration. A thorough understanding of the molecular and cellular mechanisms involved requires experimental tools that allow for inducible, tissue-specific manipulation of gene expression or signaling pathways. Therefore, we and others have recently adapted the TetON system for use in zebrafish. The TetON system facilitates temporally and spatially-controlled gene expression and we have recently used this tool to probe for tissue-specific functions of Wnt/beta–catenin signaling during zebrafish tail fin regeneration. Here we describe the workflow for using the TetON system to achieve inducible, tissue-specific gene expression in the adult regenerating zebrafish tail fin. This includes the generation of stable transgenic TetActivator and TetResponder lines, transgene induction and techniques for verification of tissue-specific gene expression in the fin regenerate. Thus, this protocol serves as blueprint for setting up a functional TetON system in zebrafish and its subsequent use, in particular for studying fin regeneration.
Involved external institutions
Germany (DE)How to cite
APA:
Wehner, D., Jahn, C., & Weidinger, G. (2015). Use of the TetOn system to study molecular mechanisms of zebrafish regeneration. Journal of Visualized Experiments, 2015(100). https://doi.org/10.3791/52756
MLA:
Wehner, Daniel, Christopher Jahn, and Gilbert Weidinger. "Use of the TetOn system to study molecular mechanisms of zebrafish regeneration." Journal of Visualized Experiments 2015.100 (2015).
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