Bone marrow concentrate for autologous transplantation in minipigs: Characterization and osteogenic potential of mesenchymal stem cells
Herten M, Grassmann JP, Sager M, Benga L, Fischer JC, Jaeger M, Wild M, Hakimi M, Jungbluth P, Betsch M (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 26
Pages Range: 34-41
Journal Issue: 1
Abstract
Autologous bone marrow plays an increasing role in the treatment of bone, cartilage and tendon healing disorders. Cell-based therapies display promising results in the support of local regeneration, especially therapies using intra-operative one-step treatments with autologous progenitor cells. In the present study, bone marrow-derived cells were concentrated in a point-of-care device and investigated for their mesenchymal stem cell (MSC) characteristics and their osteogenic potential. Bone marrow was harvested from the iliac crest of 16 minipigs. The mononucleated cells (MNC) were concentrated by gradient density centrifugation, cultivated, characterized by flow cytometry and stimulated into osteoblasts, adipocytes, and chondrocytes. Cell differentiation was investigated by histological and immunohistological staining of relevant lineage markers. The proliferation capacity was determined via colony forming units of fibroblast and of osteogenic alkalinephosphatase-positive-cells. The MNC could be enriched 3.5-fold in nucleated cell concentrate in comparison to bone marrow. Flow cytometry analysis revealed a positive signal for the MSC markers. Cells could be differentiated into the three lines confirming the MSC character. The cellular osteogenic potential correlated significantly with the percentage of newly formed bone in vivo in a porcine metaphyseal longbone defect model. This study demonstrates that bone marrow concentrate from minipigs display cells with MSC character and their osteogenic differentiation potential can be used for osseous defect repair in autologous transplantations. © Schattauer 2013.
Authors with CRIS profile
Involved external institutions
Germany (DE)How to cite
APA:
Herten, M., Grassmann, J.P., Sager, M., Benga, L., Fischer, J.C., Jaeger, M.,... Betsch, M. (2013). Bone marrow concentrate for autologous transplantation in minipigs: Characterization and osteogenic potential of mesenchymal stem cells. Veterinary and Comparative Orthopaedics and Traumatology, 26(1), 34-41. https://dx.doi.org/10.3415/VCOT-11-11-0165
MLA:
Herten, Monika, et al. "Bone marrow concentrate for autologous transplantation in minipigs: Characterization and osteogenic potential of mesenchymal stem cells." Veterinary and Comparative Orthopaedics and Traumatology 26.1 (2013): 34-41.
BibTeX: Download