The metabolic profile of reconstituting T-cells, NK-cells, and monocytes following autologous stem cell transplantation and its impact on outcome

Richter S, Böttcher M, Mackensen A, Ullrich E, Jacobs B, Mougiakakos D, Völkl S (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 12

Article Number: 11406

Journal Issue: 1

DOI: 10.1038/s41598-022-15136-3

Abstract

Previous studies indicated a role of the reconstituting immune system for disease outcome upon high-dose chemotherapy (HDCT) and autologous stem cell transplantation (auto-SCT) in multiple myeloma (MM) and lymphoma patients. Since immune cell metabolism and function are closely interconnected, we used flow-cytometry techniques to analyze key components and functions of the metabolic machinery in reconstituting immune cells upon HDCT/auto-SCT. We observed increased proliferative activity and an upregulation of the glycolytic and fatty acid oxidation (FAO) machinery in immune cells during engraftment. Metabolic activation was more pronounced in T-cells of advanced differentiation stages, in CD56^bright NK-cells, and CD14⁺⁺CD16⁺ intermediate monocytes. Next, we investigated a potential correlation between the immune cells’ metabolic profile and early progression or relapse in lymphoma patients within the first twelve months following auto-SCT. Here, persistently increased metabolic parameters correlated with a rather poor disease course. Taken together, reconstituting immune cells display an upregulated bioenergetic machinery following auto-SCT. Interestingly, a persistently enhanced metabolic immune cell phenotype correlated with reduced PFS. However, it remains to be elucidated, if the clinical data can be confirmed within a larger set of patients and if residual malignant cells not detected by conventional means possibly caused the metabolic activation.

Authors with CRIS profile

Silja Richter Medizinische Fakultät Martin Böttcher Department of Medicine 5 – Haematology and Oncology Andreas Mackensen Lehrstuhl für Innere Medizin V Benedikt Jacobs Department of Medicine 5 – Haematology and Oncology Dimitrios Mougiakakos Medizinische Fakultät Simon Völkl Department of Medicine 5 – Haematology and Oncology

Involved external institutions

Goethe-Universität Frankfurt am Main

Germany (DE)

How to cite

APA:

Richter, S., Böttcher, M., Mackensen, A., Ullrich, E., Jacobs, B., Mougiakakos, D., & Völkl, S. (2022). The metabolic profile of reconstituting T-cells, NK-cells, and monocytes following autologous stem cell transplantation and its impact on outcome. Scientific Reports, 12(1). https://doi.org/10.1038/s41598-022-15136-3

MLA:

Richter, Silja, et al. "The metabolic profile of reconstituting T-cells, NK-cells, and monocytes following autologous stem cell transplantation and its impact on outcome." Scientific Reports 12.1 (2022).

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