Visualization of superparamagnetic nanoparticles in vascular tissue using XμCT and histology

Tietze R, Rahn H, Schreiber E, Mann J, Odenbach S, Alexiou C, Lyer S (2011)

Publication Type: Journal article

Publication year: 2011

Journal

Histochemistry and Cell Biology Springer Verlag (Germany)

Book Volume: 135

Pages Range: 153-158

Journal Issue: 2

DOI: 10.1007/s00418-011-0780-8

Abstract

In order to increase the dose of antineoplastic agents in the tumor area, the concept of magnetic drug targeting (MDT) has been developed. Magnetic nanoparticles consisting of iron oxide and a biocompatible cover layer suspended in an aqueous solution (ferrofluid) serve as carriers for chemotherapeutics being enriched by an external magnetic field after intra-arterial application in desired body compartments (i.e., tumor). We established an ex vivo model to simulate in vivo conditions in a circulating system consisting of magnetic iron oxide nanoparticles passing an intact bovine artery and being focused by an external magnetic field to study their distribution in the vessel. Micro-computed X-ray tomography (XμCT) and histology can elucidate the arrangement of these particles after application. XμCT-analysis has been performed on arterial sections after MDT in order to determine the distribution of the nanoparticles. These measurements have been carried out with a cone X-ray source and corresponding histological sections were stained with Prussian blue. It could be shown that combining XμCT and histology offers the opportunity for a better understanding of the mechanisms of nanoparticle deposition in the vascular system after MDT. © 2011 Springer-Verlag.

Authors with CRIS profile

Rainer Tietze
Stefan Lyer

Involved external institutions

Technische Universität Dresden DE Germany (DE) Else Kröner-Fresenius-Zentrum für Ernährungsmedizin DE Germany (DE)

How to cite

APA:

Tietze, R., Rahn, H., Schreiber, E., Mann, J., Odenbach, S., Alexiou, C., & Lyer, S. (2011). Visualization of superparamagnetic nanoparticles in vascular tissue using XμCT and histology. Histochemistry and Cell Biology, 135(2), 153-158. https://dx.doi.org/10.1007/s00418-011-0780-8

MLA:

Tietze, Rainer, et al. "Visualization of superparamagnetic nanoparticles in vascular tissue using XμCT and histology." Histochemistry and Cell Biology 135.2 (2011): 153-158.

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