The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations

Schicht M, Riedlova K, Kukulka M, Li W, Scheer A, Garreis F, Jacobi C, Paulsen F, Cwiklik L, Bräuer L (2022)


Publication Type: Journal article

Publication year: 2022

Journal

Book Volume: 23

Journal Issue: 10

DOI: 10.3390/ijms23105783

Abstract

The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film.

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APA:

Schicht, M., Riedlova, K., Kukulka, M., Li, W., Scheer, A., Garreis, F.,... Bräuer, L. (2022). The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations. International Journal of Molecular Sciences, 23(10). https://doi.org/10.3390/ijms23105783

MLA:

Schicht, Martin, et al. "The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations." International Journal of Molecular Sciences 23.10 (2022).

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