Reconstitution of EBV-directed T cell immunity by adoptive transfer of peptide-stimulated T cells in a patient after allogeneic stem cell transplantation for AITL

Cobo MFL, Ritter J, Gary R, Seitz V, Mautner J, Aigner M, Völkl S, Schaffer S, Moi S, Seegebarth A, Bruns H, Rösler W, Amann KU, Büttner-Herold M, Hennig S, Mackensen A, Hummel M, Moosmann A, Gerbitz A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

PLoS Pathogens Public Library of Science

Book Volume: 18

Article Number: e1010206

Journal Issue: 4

DOI: 10.1371/journal.ppat.1010206

Abstract

Reconstitution of the T cell repertoire after allogeneic stem cell transplantation is a long and often incomplete process. As a result, reactivation of Epstein-Barr virus (EBV) is a frequent complication that may be treated by adoptive transfer of donor-derived EBV-specific T cells. We generated donor-derived EBV-specific T cells by stimulation with peptides representing defined epitopes covering multiple HLA restrictions. T cells were adoptively transferred to a patient who had developed persisting high titers of EBV after allogeneic stem cell transplantation for angioimmunoblastic T-cell lymphoma (AITL). T cell receptor beta (TCRβ) deep sequencing showed that the T cell repertoire of the patient early after transplantation (day 60) was strongly reduced and only very low numbers of EBV-specific T cells were detectable. Manufacturing and in vitro expansion of donor-derived EBV-specific T cells resulted in enrichment of EBV epitope-specific, HLA-restricted T cells. Monitoring of T cell clonotypes at a molecular level after adoptive transfer revealed that the dominant TCR sequences from peptide-stimulated T cells persisted long-term and established an EBV-specific TCR clonotype repertoire in the host, with many of the EBV-specific TCRs present in the donor. This reconstituted repertoire was associated with immunological control of EBV and with lack of further AITL relapse.

Authors with CRIS profile

Simon Völkl Department of Medicine 5 – Haematology and Oncology Heiko Bruns Department of Medicine 5 – Haematology and Oncology Wolf Rösler Department of Medicine 5 – Haematology and Oncology Kerstin Ute Amann Department of Nephropathology Maike Büttner-Herold Medizinische Fakultät Andreas Mackensen Lehrstuhl für Innere Medizin V

Involved external institutions

HS Diagnomics GmbH DE Germany (DE) Charité - Universitätsmedizin Berlin DE Germany (DE) Klinikum der Universität München DE Germany (DE) University of Toronto CA Canada (CA)

How to cite

APA:

Cobo, M.F.L., Ritter, J., Gary, R., Seitz, V., Mautner, J., Aigner, M.,... Gerbitz, A. (2022). Reconstitution of EBV-directed T cell immunity by adoptive transfer of peptide-stimulated T cells in a patient after allogeneic stem cell transplantation for AITL. PLoS Pathogens, 18(4). https://doi.org/10.1371/journal.ppat.1010206

MLA:

Cobo, María Fernanda Lammoglia, et al. "Reconstitution of EBV-directed T cell immunity by adoptive transfer of peptide-stimulated T cells in a patient after allogeneic stem cell transplantation for AITL." PLoS Pathogens 18.4 (2022).

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