Schwartz C, Vöhringer D (2020)
Publication Type: Journal article
Publication year: 2020
Book Volume: 2163
Pages Range: 367-375
DOI: 10.1007/978-1-0716-0696-4_29
Here, we describe how murine basophils can be detected in vivo by flow cytometry and immunofluorescence staining. Basophils constitute a homogeneous population of CD4-CD19-CD49b+IgE+ cells in flow cytometric analysis. When IgE levels are low, one can also use anti-FcεRI or anti-CD200R3 antibodies instead of anti-IgE. For immunofluorescence staining, we use an anti-Mcpt8 antibody since Mcpt8 is a specific marker for murine basophils. We describe how to prepare the tissue to cut cryo-sections and how to perform the staining using a tyramide-based amplification kit.
APA:
Schwartz, C., & Vöhringer, D. (2020). Identification of Murine Basophils by Flow Cytometry and Histology. Methods in Molecular Biology, 2163, 367-375. https://doi.org/10.1007/978-1-0716-0696-4_29
MLA:
Schwartz, Christian, and David Vöhringer. "Identification of Murine Basophils by Flow Cytometry and Histology." Methods in Molecular Biology 2163 (2020): 367-375.
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